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Journal: Breast Cancer Research : BCR
Article Title: Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility
doi: 10.1186/bcr2762
Figure Lengend Snippet: RUNX2 silencing reduces migration of metastatic MDA-MB-231 breast cancer cells . (A) Diminished expression of RUNX2 protein levels was observed by western blotting in lysates from MDA-MB-231 treated with siRUNX2. ( B ) Wound healing assay of MDA-MB-231 cells treated with siRUNX2 or a non-silencing RNA (AllStar Control) at five different time points. (C) Cell counts were performed to determine the number of cells migrating to the scratch zone after 24 h for MDA-MB-231 cells treated with siRUNX2 ( n = 180 cells) or non-silencing AllStar Control ( n = 242 cells). (D) Total percent closure was determined as a time-course after initiating the scratch. Control MDA-MB-231 cells transfected with AllStar Control (88%) achieved 29% percent closure, while cells treated with siRUNX2 (59%) exhibited reduced wound closure. Asterisk indicates statistical significance between siRUNX2 and control treatment ( P < 0.01 based on ANCOVA test). Error bars represent standard deviation.
Article Snippet: Analysis of
Techniques: Migration, Expressing, Western Blot, Wound Healing Assay, Transfection, Standard Deviation
Journal: Breast Cancer Research : BCR
Article Title: Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility
doi: 10.1186/bcr2762
Figure Lengend Snippet: RUNX2 expression increases cell migration in non-metastatic MCF7 breast cancer cells . (A) Western blot analysis using a RUNX2 antibody and/or c-Myc epitope tag in MCF7 cells transfected with a Myc-RUNX2 expression vector. Levels of β-actin were used as a control for protein loading) (B) Images from a wound healing assay with MCF7 cells transfected with empty expression vector (pcDNA3 control) or a plasmid expressing Myc-RUNX2 at various time points (in hours). (C) Quantification of total percent closure of control MCF7 cells (pcDNA3; 34%) and Myc-RUNX2 expressing MCF7 cells (41%) revealed that RUNX2 expressing increases cell motility. Asterisk indicates statistical significance between Myc-RUNX2 and control treatment ( P < 0.05 based on ANCOVA test). Error bars represent standard deviation.
Article Snippet: Analysis of
Techniques: Expressing, Migration, Western Blot, Transfection, Plasmid Preparation, Wound Healing Assay, Standard Deviation